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M9490629.TXT
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1994-09-24
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Document 0629
DOCN M9490629
TI Evidence that levels of the dimeric cellular transcription factor CP2
play little role in the activation of the HIV-1 long terminal repeat in
vivo or following superinfection with herpes simplex virus type 1.
DT 9411
AU Zhong F; Swendeman SL; Popik W; Pitha PM; Sheffery M; Molecular Biology
Program, Cornell University, Memorial; Sloan-Kettering Cancer Center,
New York, New York 10021.
SO J Biol Chem. 1994 Aug 19;269(33):21269-76. Unique Identifier : AIDSLINE
MED/94342299
AB The dimeric transcription factor CP2 binds a sequence element found near
the transcription start site of the human immunodeficiency virus (HIV-1)
long terminal repeat. Several groups have suggested that cellular
factors binding this element might play a role in modulating HIV-1
promoter activity in vivo. For example, induction of latent HIV-1 gene
expression in response to superinfection by herpes simplex virus type 1
(HSV-1) or cytomegalovirus is thought to be mediated, in part, by
factors binding the CP2 site. In this report we began to examine
directly the relationship between CP2 and expression of the HIV-1
promoter. First, we tested what effect HSV-1 infection of T cells had on
the cellular levels of CP2. The results showed that HSV-1 infection led
to a significant reduction in the level of CP2 DNA binding activity and
protein within 20 h. Next, we tested the effect of overexpressing either
the wild-type factor or a dominant negative variant of CP2 on HIV-1
promoter activity in vivo. The results showed that CP2 had little effect
or slightly repressed HIV-1 promoter activity in vivo. In addition,
these expression constructs had little effect on the induction of HIV-1
promoter activity elicited by HSV-1 infection.
DE Amino Acid Sequence Base Sequence Binding Sites Cells, Cultured
DNA-Binding Proteins/*METABOLISM DNA, Viral/METABOLISM Herpesvirus 1,
Human/*METABOLISM *HIV Long Terminal Repeat HIV-1/*GENETICS Molecular
Sequence Data NF-kappa B/METABOLISM *Promoter Regions (Genetics)
Sequence Homology, Amino Acid Support, Non-U.S. Gov't Support, U.S.
Gov't, P.H.S. T-Lymphocytes/MICROBIOLOGY Transcription
Factors/*METABOLISM JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).